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Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin
$400.00 - $3,780.00
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Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Species Reactivity
Mouse
Target
CD28
Concentration
1.0 - 5.0 mg/ml
Isotype
Syrian Hamster IgG
Host
Syrian Hamster
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Applications
Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen)
Storage
anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
20 mM Histidine pH 6.0 containing 150 mM NaCl. NOTE: A buffer exchange into PBS for conjugation and MES for functional assays may be required depending on your application.
Purification Method
This monoclonal antibody was purified using Protein A
Specificity
Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.
Shipping Conditions
Blue ice
Background
CD28 is the principal co-stimulatory receptor on T cells, delivering the second signal needed for full activation, proliferation and survival. Clone 37.51 is a Syrian hamster IgG monoclonal antibody to mouse CD28, validated for immunoprecipitation, Western blot, blocking, functional assays, flow cytometry and frozen IHC. Anti-mouse CD28 37.51 is supplied in a low-endotoxin format suitable for in vivo use and produced in scalable milligram (mg) to gram (g) quantities for bulk and high-throughput laboratories. As a non-therapeutic, research-use-only (RUO) reagent, 37.51 provides dependable, reproducible results for studies of T-cell co-stimulation in the mouse.
Clone
37.51
Uniprot
P31041
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Antigen Distribution
Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells. Clone 37.51 antibody has been shown to stimulate the proliferation and cytokine production by activated T and
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Aggregation
Aggregation level ≤ 5%
Antibodies Against The Same Target
Related Antibody
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