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Anti-Mouse PD-L1 (10F.9G2) In Vivo Antibody - Low Endotoxin

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ICH1086

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$150.00 - $23,460.00

Anti-Mouse PD-L1 (10F.9G2) In Vivo Antibody - Low Endotoxin

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Product Specific Citations

Majewska, J. et al. p16-dependent increase of PD-L1 stability regulates immunosurveillance of senescent cells. Nat Cell Biol, 2024, 26, 1336–1345.

Chen et al. NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer. Adv. Sci, 2023, 10, 2302705.

Lin et al. Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice. Cell Reports, 2023, 42, 7112812

Qiu et al. PPP2R2A insufficiency enhances PD-L1 immune checkpoint blockade efficacy in lung cancer through cGAS-STING activation. J Clin Invest. 2025, 136(4):e193354.

Species Reactivity

Mouse

Target

PD-L1

Concentration

>1mg/ml

Isotype

Rat IgG2b Kappa

Host

Rat

Related Antibody

Anti-PD-L1 In Vivo Antibody - Ultra Low Endotoxin [10F.9G2] (ICH1086UL)

Antibodies Against The Same Target

Aggregation

Aggregation level ≤ 5%

Immunogen

Unknown

Antigen Distribution

The CD274 antigien is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Uniprot

Q9EP73

Gene Id

60533

Rrid

RRID:AB_3075574

Shipping Conditions

Blue ice

Background

This antibody is the widely used rat anti-mouse PD-L1 clone 10F.9G2 (rat IgG2b, kappa), supplied in a low-endotoxin, RUO format intended for in vivo and functional work in mouse models. It recognizes mouse PD-L1 (CD274, UniProt Q9EP73) and is applied to flow cytometry, blocking assays, IHC on frozen sections, immunofluorescence, and functional/cell-based assays. Its established utility as a checkpoint-blocking reagent, combined with a low endotoxin burden (research grade under 1 EU/mg; ultra-low under 0.5 EU/mg) and availability in bulk milligram-to-gram quantities, makes it well suited to repeat-dose in vivo tumour immunology and T cell activation studies where endotoxin contamination would confound immune readouts. The rat IgG2b backbone is a common choice for functional PD-L1 blockade in mice, and researchers typically identify it by the 10F.9G2 clone designation. All material is for research use only, and this text is provided as guidance for ichor.bio to review before publishing. PD-L1 (programmed death-ligand 1; CD274, B7-H1) is a type I transmembrane glycoprotein of the B7 family and a principal ligand for the inhibitory receptor PD-1. Engagement of PD-1 by PD-L1 delivers a co-inhibitory signal that dampens T cell receptor signalling, curbing proliferation, cytokine production, and effector function, and contributes to peripheral tolerance and T cell exhaustion. PD-L1 is expressed constitutively on some antigen-presenting cells and is strongly upregulated on many cell types, including tumour cells and stroma, in response to inflammatory cytokines such as IFN-gamma. PD-L1 also engages CD80 (B7-1). By co-opting this pathway, tumours evade immune attack, which is why blockade of the PD-1/PD-L1 axis is a central strategy in cancer immunotherapy and a frequent target in preclinical mouse studies.

Other Names

Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, 10F 9G2, 10F-9G2, 10F9G2

Clone

10F.9G2

Specificity

Anti-PD-L1 In Vivo Antibody - Low Endotoxin (10F.9G2) recognizes an epitope on Mouse CD274

Purification Method

This monoclonal antibody was purified using Protein G

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Storage

anti-PD-L1 In Vivo Antibody - Low Endotoxin (10F.9G2) is stable when stored at 2-8°C. Do not freeze.

Applications

Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays

Application Notes

Each investigator should determine their own optimal working dilution for specific applications.

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Bio X Cell Code

BE0101, BP0101

Suggested H1

Anti-Mouse PD-L1/Cd274 (10F.9G2) In Vivo Antibody - Low Endotoxin

Faq (q&a)

Q: Is clone 10F.9G2 suitable for in vivo use?

A: Yes — 10F.9G2 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo PD-L1 studies as well as in vitro work.

Q: Can I order 10F.9G2 in bulk or gram quantities?

A: Yes, this anti-mouse PD-L1 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 10F.9G2 target?

A: It is an anti-mouse PD-L1 monoclonal antibody that binds PD-L1 and is used in functional and blocking studies; for research use only (RUO).

More Information

Bio X Cell:

ICH1086 is up to 35% cheaper for academia & non-profits and up to 58% cheaper for industry than the equivalent product low endotoxin product (BE0101) and up to 36% cheaper for academics & up to 59% cheaper for industry for the ultra-low endotoxin version (BP0101) from Bio X Cell.

Product Benefits:

ichorbio's anti-PD-L1 In Vivo Antibody - Low Endotoxin (10F.9G2) is manufactured in a cGMP compliant facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Size:

ichorbio's 10F.9G2 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-PD-L1 10F.9G2 clone - please contact us for pricing.

Immunogen:

Unknown

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus

Isotype Control:

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Mouse anti-mouse version:

Mouse anti-Mouse PD-L1 Antibody (10F.9G2) [ICH1183]

Immunofluorescence of frozen tissue sections

Sample: Frozen sections of tumor tissues from tumor bearing C57BL / 6 mice (inoculated with LLC cells) Protocol: 1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose; 2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS; 3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h; 4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio PD-L1 antibody clone 10F.9G2 (low) 1μg/ml, Final concentration (high) 5μg/ml. Positive signals were detected at both high and low concentrations 5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h Details of secondary antibody: Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody - Jackson Immunoresearch Labs Cat# 112-605-062 - Conc. 7.5μg/ml. 6. Washed by PBST at least 6 times； 7. Add fluorescence decay resistant medium, seal slice; 8. Detected by the laser scanning confocal microscope. Scale bar in the IF figure is 50 μM. Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University

Alternative Names: