Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Low Endotoxin

Mine et al. TYK2 signaling promotes the development of autoreactive CD8+ cytotoxic T lymphocytes and type 1 diabetes. Nature Communications, 2024, 15, Article number: 1337

Mouse

IFN beta

>1mg/ml

Mouse IgG2a

Mouse

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin [HDB-4A7] (ICH1110UL)

Anti-Mouse IFN Beta (MIB-5E9.1) In Vivo Antibody - Low Endotoxin (ICH1124)

Aggregation level ≤ 5%

Plasmid DNA encoding murine IFNb

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01575

15977

RRID:AB_2921488

Blue ice

This is clone HDB-4A7, a mouse monoclonal antibody of the IgG2a isotype raised against mouse interferon beta (IFN beta). Because the immunogen, host, and target are all murine, the antibody is well suited to in-vivo neutralization studies in mouse models without the confounding anti-species immune responses that can accompany rat or hamster reagents used long term. HDB-4A7 recognizes and functionally blocks bioactive mouse IFN beta, and is validated for Western blot, blocking, and functional (neutralization) assays, making it useful both for detecting the cytokine and for interrogating its biological role in vivo. The antibody is supplied low-endotoxin (research grade below 1 EU/mg, ultra-low-endotoxin option below 0.5 EU/mg) and in bulk milligram-to-gram quantities, so that repeated dosing across treatment cohorts is feasible while minimizing endotoxin-driven artefacts that would otherwise confound type I interferon readouts. It is provided for research use only (RUO) and is intended as a tool for controlled laboratory investigation rather than any diagnostic or therapeutic application. Interferon beta (IFN beta, gene Ifnb1; UniProt P01575 in mouse) is a type I interferon and a central cytokine of innate immunity. It is induced rapidly upon sensing of viral and microbial nucleic acids and other danger signals through pattern-recognition pathways, and unlike the multiple IFN alpha subtypes it is encoded by a single gene. Secreted IFN beta signals in an autocrine and paracrine manner by binding the shared type I interferon receptor (IFNAR1/IFNAR2), triggering JAK-STAT signaling and transcription of interferon-stimulated genes that establish an antiviral state. Beyond antiviral defense, IFN beta shapes immune responses by influencing dendritic cell maturation, T cell and NK cell activity, and antitumor immunity, and it has context-dependent roles in autoimmunity and chronic inflammation. Because type I interferon signaling can be either protective or pathogenic depending on setting, neutralizing IFN beta specifically is a valuable way to dissect its distinct contribution.

IFN-b, IFN-beta, Type-1 interferon, Interferon beta, HDB 4A7, HDB4A7

HDB-4A7

Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Blocking, Functional Assays

Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Low Endotoxin

Q: Is clone HDB-4A7 suitable for in vivo use?

A: Yes — HDB-4A7 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo IFN beta studies as well as in vitro work.

Q: Can I order HDB-4A7 in bulk or gram quantities?

A: Yes, this anti-mouse IFN beta antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone HDB-4A7 target?

A: It is an anti-mouse IFN beta monoclonal antibody that binds IFN beta and is used in functional and blocking studies; for research use only (RUO).