Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin

Mouse

CD80

>1mg/ml

Armenian Hamster IgG

Armenian Hamster

Anti-CD80 In Vivo Antibody - Ultra Low Endotoxin [16-10A1] (ICH1037UL)

Anti-Mouse CD80 (RM80) In Vivo Antibody - Low Endotoxin (ICH1276), Galiximab Biosimilar - Research Grade (ICH5196)

Aggregation level ≤ 5%

Purified Recombinant Mouse CD80 (>98%)

Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q00609

Blue ice

This antibody targets mouse CD80 (B7-1) using the well-established clone 16-10A1, an Armenian hamster IgG raised against mouse CD80. The clone is widely used for detecting and functionally blocking CD80 and recognizes the native cell-surface molecule, making it suitable for flow cytometry, immunoprecipitation, Western blot, IHC on frozen sections, and costimulatory blocking studies. Because CD80 is a key ligand for CD28 and CTLA-4, 16-10A1 is frequently applied in T-cell costimulation and tolerance research, including in-vivo experiments where interruption of the B7:CD28/CTLA-4 axis is of interest. This preparation is produced for in-vivo and functional applications: it is supplied at low endotoxin levels appropriate for research use, is available in bulk milligram-to-gram quantities, and is intended for research use only. The Armenian hamster IgG format and defined clone make it a consistent, lot-to-lot reproducible reagent for immunology, tumour immunology, and transplantation models where reliable CD80 recognition and costimulatory pathway modulation are required. CD80 (B7-1) is a type I transmembrane glycoprotein of the immunoglobulin superfamily expressed primarily on activated antigen-presenting cells, including dendritic cells, activated B cells, and macrophages. Together with its homologue CD86 (B7-2), CD80 provides the second, costimulatory signal required for productive T-cell activation. It engages two counter-receptors with opposing outcomes: binding to CD28 delivers a stimulatory signal that promotes T-cell proliferation, IL-2 production, and survival, whereas binding to CTLA-4, which has higher affinity, transmits inhibitory signals that restrain T-cell responses and contribute to peripheral tolerance. CD80 also interacts with PD-L1 in cis, modulating checkpoint signalling. Through these interactions, CD80 sits at a central node governing the balance between immune activation and tolerance, and is studied extensively in autoimmunity, transplantation, and antitumour immunity.

Activation B7-1 antigen, B7, 16 10A1, 1610A1

16-10A1

Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 16-10A1 suitable for in vivo CD80 blocking?

A: Yes, 16-10A1 is supplied in a low-endotoxin, in vivo-grade format suitable for CD80 blocking and functional studies as well as in vitro work.

Q: Can I order clone 16-10A1 anti-CD80 in bulk?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput in vivo experiments.

Q: What does clone 16-10A1 target?

A: 16-10A1 is an anti-mouse CD80 (B7-1) monoclonal antibody used to study costimulation; for research use only.