Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Mouse

CD8 beta

>1mg/ml

Rat IgG1

Rat

Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin [53-5.8] (ICH1046UL)

Aggregation level ≤ 5%

Mouse thymus or spleen

Majority of thymocytes, cytotoxic T cell subsets

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P10300

Blue ice

This is a rat anti-mouse CD8 beta monoclonal antibody, clone 53-5.8, of the rat IgG1 isotype, raised in rat against the mouse CD8 beta chain (UniProt P10300). The clone recognizes an epitope on the CD8 beta subunit rather than the more commonly targeted CD8 alpha chain, which distinguishes it from depletion-oriented clones such as 53-6.7. Provided as a low-endotoxin (<1 EU/mg research grade; ultra-low <0.5 EU/mg options), azide-free preparation supplied in bulk milligram-to-gram quantities, it is formulated for in-vivo administration and functional work in mice where endotoxin load and aggregate content matter. The antibody validates across Western blot, functional assays, flow cytometry, immunocytochemistry, IHC on frozen sections, and immunoprecipitation, making it suitable for pairing in-vivo intervention studies with downstream immunophenotyping and tissue analysis. It is intended for research use only (RUO) and is not for diagnostic or therapeutic use. Researchers typically source this clone by name (53-5.8) when they need to interrogate the CD8 beta co-receptor specifically in mouse cytotoxic T cell biology. CD8 is the co-receptor that defines cytotoxic T lymphocytes and marks MHC class I-restricted recognition. On most conventional cytotoxic T cells CD8 is expressed as an alpha-beta heterodimer, whereas the alpha-alpha homodimer appears on subsets such as intraepithelial lymphocytes and some innate-like cells. The beta chain is not merely structural: it enhances co-receptor function by promoting association with the Lck kinase, positioning CD8 within the immunological synapse, and stabilizing the interaction between the T cell receptor and peptide-MHC class I. Because the alpha-beta heterodimer is far more efficient than alpha-alpha at supporting antigen recognition and TCR signaling, targeting the beta chain specifically allows investigators to probe conventional CD8 alpha-beta T cell function while distinguishing it from alpha-alpha-bearing populations. CD8 beta thus serves as a marker and functional modulator central to cytotoxic responses against intracellular pathogens and tumors.

Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3, 53 5.8, 53-5 8, 53-5-8, 53-58, 535.8, 5358

53-5.8

Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1

This monoclonal antibody was purified using Protein G

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 53-5.8 suitable for in vivo use?

A: Yes — 53-5.8 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD8 beta studies as well as in vitro work.

Q: Can I order 53-5.8 in bulk or gram quantities?

A: Yes, this anti-mouse CD8 beta antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 53-5.8 target?

A: It is an anti-mouse CD8 beta monoclonal antibody that binds CD8 beta and is used in functional and blocking studies; for research use only (RUO).