Anti-Mouse CD44 (IM7) In Vivo Antibody - Low Endotoxin

Mouse

CD44

>1mg/ml

Rat IgG2b

Rat

Anti-CD44 In Vivo Antibody - Ultra Low Endotoxin [IM7] (ICH1115UL)

Bivatuzumab Biosimilar - Research Grade (ICH5405)

Aggregation level ≤ 5%

Dexamethasone-induced myeloid leukemia M1 cells

The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reac

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P15379

12505

RRID:AB_2921491

Blue ice

This is the IM7 clone, a rat IgG2b monoclonal raised against mouse CD44. IM7 is one of the most widely used anti-CD44 antibodies and reacts with an epitope shared by all known mouse CD44 isoforms (the standard form and CD44 variant splice forms), making it a broad pan-CD44 reagent. The rat IgG2b format is well suited to in-vivo and functional work in mice, where cross-reactivity and Fc handling are important considerations. This preparation is offered as a low-endotoxin, in-vivo-ready formulation (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and is available in bulk milligram-to-gram quantities to support repeat dosing and multi-cohort studies. It is validated across a range of applications including flow cytometry, CyTOF, immunoprecipitation, Western blot, frozen-section IHC, ELISA, and functional assays, and is commonly used to track and manipulate CD44-expressing populations such as activated and memory T cells. For research use only. All content here is guidance for ichorbio to review before publishing. CD44 is a widely expressed single-pass transmembrane glycoprotein and the principal cell-surface receptor for hyaluronan, though it also binds osteopontin, collagens, matrix metalloproteinases, and other extracellular matrix components. Extensive alternative splicing of variant exons and heavy post-translational glycosylation generate a large family of isoforms, from the ubiquitous standard form (CD44s) to tissue- and activation-restricted variant forms (CD44v). Through hyaluronan engagement and links to the cytoskeleton, CD44 contributes to cell adhesion, migration, and signaling. In immunology it is a classic marker of lymphocyte activation and memory: naive T cells are CD44-low, whereas antigen-experienced effector and memory T cells upregulate CD44, and CD44 participates in leukocyte rolling, extravasation, and homing. CD44 is also implicated in tumor invasion, metastasis, and cancer stem-cell biology, which is why it is studied both as a phenotypic marker and as a functional target.

Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I

IM7

Anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186

This monoclonal antibody was purified using Protein G

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen), ELISA, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0039

Anti-Mouse CD44/ECMR-III (IM7) In Vivo Antibody - Low Endotoxin

Q: Is clone IM7 suitable for in vivo use?

A: Yes — IM7 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD44 studies as well as in vitro work.

Q: Can I order IM7 in bulk or gram quantities?

A: Yes, this anti-mouse CD44 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone IM7 target?

A: It is an anti-mouse CD44 monoclonal antibody that binds CD44 and is used in functional and blocking studies; for research use only (RUO).