Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Mouse

CD3e

>1mg/ml

Syrian Hamster IgG2a Kappa

Syrian Hamster

Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL)

Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin (ICH1041), Bulk Anti-Human CD3 (OKT-3) Antibody (ICH1257), Muromonab Biosimilar - Research Grade (ICH5024), Teplizumab Biosimilar - Research Grade (ICH5101), Foralumab Biosimilar - Research Grade (ICH5156), Otelixizumab Biosimilar - Research Grade (ICH5171), Dafsolimab Biosimilar - Research Grade (ICH5460), Emirodatamab Biosimilar - Research Grade (ICH5520), Visilizumab Biosimilar - Research Grade (ICH5982)

Aggregation level ≤ 5%

Mouse T-cell receptor

The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P22646

Blue ice

Clone 500A2 is a Syrian hamster IgG2a kappa monoclonal antibody raised against mouse CD3e, the epsilon subunit of the CD3/T-cell receptor (TCR) signalling complex. Because it recognises a widely conserved component of the TCR complex on essentially all mature ab T cells, 500A2 is a standard tool for engaging, activating and modulating murine T cells in functional and in vivo settings, and is also validated for flow cytometry and IHC on frozen sections. The hamster IgG2a format and pan-T reactivity make it well suited to studies of T-cell activation, TCR signalling, tolerance induction and T-cell dynamics in immunocompetent mice. This preparation is manufactured for in vivo and functional research: it is supplied at low endotoxin levels (research grade <1 EU/mg; ultra-low <0.5 EU/mg) to minimise confounding innate stimulation, is available in bulk mg-to-gram quantities to support dosing across cohorts and repeat experiments, and is provided for research use only (RUO). Purity, aggregate control and low endotoxin are particularly important for anti-CD3 antibodies given their potency at engaging the TCR complex. CD3e (CD3 epsilon) is an invariant, non-polymorphic subunit of the CD3 complex that, together with CD3 gamma, CD3 delta and the zeta (CD247) homodimer, assembles with the clonotypic TCR alpha/beta heterodimer to form the complete T-cell receptor complex. CD3e itself does not bind antigen; instead it is essential for TCR assembly, surface expression and, critically, for transducing activation signals. Its cytoplasmic tail carries immunoreceptor tyrosine-based activation motifs (ITAMs) that, upon TCR engagement, are phosphorylated by Lck and recruit ZAP-70, initiating the downstream cascade (LAT, PLCg1, calcium flux, NFAT/NF-kB/AP-1) that drives T-cell activation, proliferation and effector differentiation. CD3e is expressed on essentially all mature ab and gd T cells and thymocytes, making it a pan-T-cell marker. Antibodies to CD3e can cluster the TCR complex and deliver activating signals independent of peptide-MHC, which underlies their use as polyclonal T-cell stimulators and immunomodulators.

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

500A2

Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Functional Assays, Flow Cytometry, IHC (Frozen)

Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 500A2 suitable for in vivo use?

A: Yes — 500A2 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD3e studies as well as in vitro work.

Q: Can I order 500A2 in bulk or gram quantities?

A: Yes, this anti-mouse CD3e antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 500A2 target?

A: It is an anti-mouse CD3e monoclonal antibody that binds CD3e and is used in functional and blocking studies; for research use only (RUO).