Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin

Mouse

CD3e

>1mg/ml

Armenian Hamster IgG

Armenian Hamster

Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [145-2C11] (ICH1041UL)

Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin (ICH1040), Bulk Anti-Human CD3 (OKT-3) Antibody (ICH1257), Muromonab Biosimilar - Research Grade (ICH5024), Teplizumab Biosimilar - Research Grade (ICH5101), Foralumab Biosimilar - Research Grade (ICH5156), Otelixizumab Biosimilar - Research Grade (ICH5171), Dafsolimab Biosimilar - Research Grade (ICH5460), Emirodatamab Biosimilar - Research Grade (ICH5520), Visilizumab Biosimilar - Research Grade (ICH5982)

Aggregation level ≤ 5%

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P22646

12501

Blue ice

This is the anti-mouse CD3e antibody, clone 145-2C11, an Armenian hamster IgG raised against mouse CD3 epsilon. 145-2C11 is one of the most widely used and best-characterized anti-mouse CD3 clones, valued for its ability to engage the CD3/TCR complex on essentially all mature T cells across common mouse strains. Because it binds a pan-T-cell target and can both activate and modulate T cells in vivo, it is a standard reagent for functional immunology, T-cell biology, and tolerance/transplantation studies, as well as for identifying and enumerating T cells by flow cytometry. It also supports IHC on frozen sections, immunoprecipitation, and Western blot. This preparation is produced for in-vivo and functional applications: supplied as low-endotoxin, research-grade material suitable for injection into mice, available in bulk milligram-to-gram quantities, and offered strictly for research use only (RUO). The hamster IgG format and validated reactivity make it a flexible tool for depletion, T-cell modulation, and antigen-detection workflows in mouse models. CD3 epsilon (CD3e) is a signaling subunit of the CD3 complex, which assembles with the T-cell receptor (TCR) alpha/beta (or gamma/delta) heterodimer to form the TCR-CD3 complex on the surface of T lymphocytes. CD3 comprises the CD3 gamma, delta, epsilon, and zeta chains; the epsilon chain pairs with gamma and delta and contributes immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic tail. Following antigen recognition by the TCR, ITAM phosphorylation by Lck initiates the signaling cascade that drives T-cell activation, proliferation, and effector differentiation. Because CD3e is expressed on all mature T cells and is essential for surface TCR assembly and signal transduction, it is both a definitive pan-T-cell marker and a key node for therapeutically modulating T-cell activity.

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e, 145 2C11, 1452C11

145-2C11

Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking

Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0001-1, BP0001-1

Anti-CD3e/1452C11 (145-2C11) In Vivo Antibody - Low Endotoxin

Q: Is clone 145-2C11 suitable for in vivo use?

A: Yes — 145-2C11 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD3e studies as well as in vitro work.

Q: Can I order 145-2C11 in bulk or gram quantities?

A: Yes, this anti-mouse CD3e antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 145-2C11 target?

A: It is an anti-mouse CD3e monoclonal antibody that binds CD3e and is used in functional and blocking studies; for research use only (RUO).