ichorbio’s Recombinant Mouse CTLA-4 is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56
>97% by SDS-PAGE and analyzed by silver stain.
Measured by its ability to inhibit IL-2 production in Jurkat cells stimulated with 1 μg/ml rhB7-1/Fc in the presence of PHA (Linsley, P.S. et al., 1991, J. Exp. Med. 174:561). The expected ED50 for this effect is 0.1 – 0.4 μg/ml..
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Predicted Molecular Mass:
The predicted molecular weight of Recombinant Mouse CTLA-4 is Mr 40 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 55 kDa.
N-terminal Sequence Analysis:
Products are for research use only. Not for use in diagnostic or therapeutic procedures