ichorbio’s Recombinant Mouse CD86 is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Sf 21 Insect Cells
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.
BB7-2, B70, Ly-58, CD-86
>95% by SDS-PAGE and analyzed by silver stain.
The biological activity of Mouse B7-2 was determined by its ability to induce IL-2 secretion by human acute leukemia T cells. The expected ED50=0.05 -0.15 ug/ml in the presence of PHA.
<1.0 EU/ug as determined by the LAL method. Suitable for in vivo use.