Recombinant Mouse CD86 [ICH3072]
Product Benefits:
ichorbio’s Recombinant Mouse CD86 is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Expression Host:
Sf 21 Insect Cells
Formulation:
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Background:
CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells.
Other Names:
BB7-2, B70, Ly-58, CD-86
Purity:
>95% by SDS-PAGE and analyzed by silver stain.
Biological activity:
The biological activity of Mouse B7-2 was determined by its ability to induce IL-2 secretion by human acute leukemia T cells. The expected ED50=0.05 -0.15 ug/ml in the presence of PHA.
Endotoxin level:
<1.0 EU/ug as determined by the LAL method. Suitable for in vivo use.
Amino acid sequence:
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State of Matter:
Lyophilized.
Storage & Stability:
The predicted molecular weight of Recombinant Mouse B7-2 is Mr 51.8 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 60-65 kDa.
Predicted Molecular Mass:
The predicted molecular weight of Recombinant Mouse B7-1 is Mr 51.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 80-90 kDa.
N-terminal Sequence Analysis:
Val26
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures
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