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Sf-21 Insect Cells
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56
>90% by SDS-PAGE and analyzed by silver stain.
The biological activity of Human CTLA-4 was determined by its ability to inhibit IL-2 production by Jurkat cells stimulated with Raji cells or 1 μg/ml rhB7-1/Fc in the presence of PHA (Linsley, P.S. et al., 1991, J. Exp. Med. 174:561). The expected ED50 is typically 2 – 4 μg/ml with Raji cells or 0.1 – 0.4 μg/ml with rhB7-H1/Fc.
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Predicted Molecular Mass:
The predicted molecular weight of Recombinant Human CTLA-4 is Mr 40 kDa (monomer).
N-terminal Sequence Analysis:
Products are for research use only. Not for use in diagnostic or therapeutic procedures