In Vivo Antibody Labeling Kit CF® 750
ichorbio’s In Vivo Antibody Labeling Kit CF® 750 is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Typical near-infrared (NIR) dyes are larger in size than dyes in the visible range, resulting in low dye solubility, dye aggregation and poor fluorescence quantum yield. To overcome these problems, many near-IR dyes, such as the near-IR Alexa Fluor® dyes, DyLight® dyes and IRDyes®, are engineered to have negatively charged sulfonate groups. While sulfonation improves dye solubility and fluorescence quantum yield to some degree, it creates another serious problem: non-specific binding of the bioconjugates prepared from the dyes caused by the highly negatively charged dyes.
ichorbio’s CF® dyes have a revolutionary new approach to near-IR dye design that results in superior physical properties of the dyes without introducing an excessive amount of negative charge. Our near-IR CF® dyes are modified such that the intramolecular mobility of the dyes is restricted, which leads to higher quantum yield and better water solubility without adding excessive charge. As a result, near-IR CF® dyes are much brighter and more photostable than any other near-IR dyes. Most importantly, antibodies labeled with near-IR CF™ dyes give far better signal-to-noise ratio in immunostaining compared with antibody conjugates prepared with other commercial near-IR dyes.
This labeling kit comprises near-infra red amine-reactive CF® 750 dye and other components necessary for carrying out antibody labeling, purification, and filter sterilization for use in small animal in vivo imaging experiments.
The reactive dye has a succinimidyl ester group, which reacts with an amine group of the protein (i.e., lysine side-chain amine) to form a stable amide linkage. The dye is supplied in three separate vials, each containing sufficient dye for labeling 1-2 mg of IgG antibody. Following the labeling reaction, unconjugated dye is conveniently and rapidly removed using the provided ultrafiltration microcentrifuge vials.
- Highly water soluble
- Exceptionally bright and stable
- Superior signal to noise ratio
- Long in vivo half life
- NIR Western
- In vivo small animal imaging
- Flow cytometry & microscopy
- Super-resolution microscopy
- Photoacoustic imaging