Bulk anti-VISTA In Vivo Antibody – Low Endotoxin (13F3)
ichorbio’s anti-VISTA In Vivo Antibody – Low Endotoxin (13F3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Anti-VISTA In Vivo Antibody – Low Endotoxin (13F3) recognizes an epitope on Mouse VISTA and is also known as PD-1H and B7-H5
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
EL4 cells overexpressing mouse VISTA-RFP followed by boosting protocol with VISTA-Ig fusion protein (Accession No.: NP_001153044)
≥ 2.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-VISTA In Vivo Antibody – Low Endotoxin (13F3) is stable when stored at 2-8°C. Do not freeze.
Western Blot, Blocking
Blocking: Blocking of VISTA with clone 13F3 antibody results in delayed tumor growth in mouse models of melanoma.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.