Bulk anti-TER-119 In Vivo Antibody – Low Endotoxin (TER-119)
ichorbio’s anti-TER-119 In Vivo Antibody – Low Endotoxin (TER-119) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Anti-TER-119 In Vivo Antibody – Low Endotoxin (TER-119) recognizes an epitope on Mouse TER-119
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
TER-119 is also known as Lymphocyte antigen 76 (Ly76) and is known to associate with the 52 kDa glycophorin A protein.
Purified Recombinant Mouse TER-119 (>98%)
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
<1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-TER-119 In Vivo Antibody – Low Endotoxin (TER-119) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Flow Cytometry, Immunoprecipitation, Western Blot
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TER-119. A suggested method would be to stain cells expressing TER-119 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Goat F(ab’)2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.