ichorbio’s Anti-Mouse PD-1 Antibody (J43) – Biotin is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Anti-Mouse PD-1 Antibody (J43) – Biotin recognizes an epitope on Mouse PD-1
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Purified Recombinant Mouse PD-1 (>98%)
This Biotin conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
This Biotin conjugate is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing PD-1. A suggested method would be to stain cells expressing PD-1 with 100 µl per 1 x 10^6 cells in a total staining volume of 100 µl followed by avidin. Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.