Anti-Mouse H-2b (B8-24-3) In Vivo Antibody - Low Endotoxin

Mouse

Histone H2B

>1mg/ml

Mouse IgG1 Kappa

Mouse

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P33778;P62807;P58876;Q93079;O60814;Q99880;Q99879;Q99877;Q5QNW6;P57053

Blue ice

NOTE: written to the antibody's true target (MHC class I H-2b); see flag re: card error. Clone B8-24-3 is a mouse-derived monoclonal antibody (mouse IgG1, kappa) that recognizes the mouse MHC class I H-2b haplotype alloantigen. The hybridoma (ATCC TIB-139) was raised against H-2b-bearing cells; the antibody binds cells of the H-2 b haplotype and cross-reacts with H-2p/H-2pa, while remaining negative on d, f, j, k, q, s, v, u and w haplotypes, giving it clean haplotype-level discrimination. Because it targets a cell-surface alloantigen rather than an intracellular protein, B8-24-3 is well suited to in-vivo and functional immunology work: allorecognition and transplantation models, studies of antigen presentation to CD8+ T cells, and identification or manipulation of H-2b-positive populations in chimeras and adoptive-transfer settings. This ichor.bio preparation is supplied research-grade with low endotoxin (<1 EU/mg; ultra-low <0.5 EU/mg options), in-vivo-ready formulation, and bulk mg-to-gram quantities. For Research Use Only; not for diagnostic or therapeutic use. All content here is draft guidance for ichor.bio to review before publishing. NOTE: written to the antibody's true target, MHC class I H-2b (NOT histone H2B — see flag). H-2b is the classical MHC class I haplotype of C57BL/6 and related mouse strains, encoded at the H-2K, H-2D and H-2L loci. MHC class I molecules are heterodimers of a polymorphic heavy chain non-covalently associated with beta-2-microglobulin. They are expressed on nearly all nucleated cells and continuously present short (8-10mer) peptides derived from intracellular proteins to CD8+ cytotoxic T cells via the T-cell receptor. This surveillance pathway lets the immune system detect virally infected, transformed, or otherwise abnormal cells, and MHC class I alloantigens are central to allorecognition and graft rejection. The polymorphic residues that define the b haplotype (H-2Kb, H-2Db) shape both peptide-binding specificity and allogeneic recognition, making haplotype-specific antibodies useful tools for identifying, tracking, and functionally probing H-2b-bearing cells.

H-2B, B8 24 3, B8243

B8-24-3

Mouse H-2b

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone B8-24-3 suitable for in vivo use?

A: Yes - B8-24-3 is a low-endotoxin, in vivo-grade antibody suitable for in vivo Histone H2B studies and in vitro work.

Q: Can anti-mouse Histone H2B B8-24-3 be ordered in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone B8-24-3 target?

A: It is an anti-mouse monoclonal raised against Histone H2B used in functional research studies; for research use only.