ichorbio’s anti-Mouse CD86 Antibody (GL1) – Biotin is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4). CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.
LPS-activated CBA/Ca mouse splenic B cells
This Biotin conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
This antibody is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD86. A suggested method would be to stain cells expressing CD86 with 0.25 µg per 1 x 10^6 cells in a total staining volume of 100 µl followed by avidin. Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.