Anti-Mouse CD70 (TAN 1-7) In Vivo Antibody - Low Endotoxin

Mouse

CD70

>1mg/ml

Rat IgG2a Kappa

Rat

Anti-Mouse CD70 (FR70) In Vivo Antibody - Low Endotoxin (ICH1230), Cusatuzumab Biosimilar - Research Grade (ICH5037), Vorsetuzumab Biosimilar - Research Grade (ICH5992)

Aggregation level ≤ 5%

Recombinant mouse CD70 (extracellular domain, aa residues 41-195).

CD70 is expressed on activated T Cells, B Cells, and dendritic cells.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

O55237

Blue ice

This is a rat anti-mouse CD70 monoclonal antibody, clone TAN1-7, of the rat IgG2a kappa isotype. Rat IgG2a antibodies are frequently chosen for in vivo mouse studies because the isotype is functionally active in mice, and CD70-directed reagents are widely used to probe the CD27-CD70 costimulatory axis in models of anti-tumour immunity, chronic infection and autoimmunity. The antibody is validated for ELISA, flow cytometry, immunofluorescence and blocking applications, making it suitable for both characterising CD70 expression on immune cell subsets and for functional interference experiments. Supplied as a low-endotoxin, research-use-only preparation (research grade less than 1 EU/mg; ultra-low endotoxin option less than 0.5 EU/mg), it is intended for in vivo work where endotoxin contamination would otherwise confound immunological readouts. Bulk milligram-to-gram quantities support multi-arm cohort studies and repeat dosing. As with any in vivo reagent, users should confirm lot-specific concentration, endotoxin level and target engagement in their own hands before scaling up. CD70 (CD27 ligand; UniProt O55237) is a type II transmembrane glycoprotein of the TNF superfamily (TNFSF7) and the sole ligand for CD27 (TNFRSF7). Unlike many costimulatory ligands, CD70 expression is tightly restricted and largely inducible: it appears transiently on activated T and B lymphocytes, mature dendritic cells and NK cells following antigen or Toll-like receptor stimulation. Engagement of CD27 by CD70 delivers a costimulatory signal that promotes T-cell proliferation, effector and memory differentiation, survival and cytokine production, and it also modulates B-cell and NK-cell responses. Because its expression is normally transient and activation-dependent, aberrant or constitutive CD70 expression, seen on several tumours and in chronic inflammation, can drive sustained CD27 signalling, contributing to T-cell exhaustion, regulatory T-cell expansion and immune dysregulation. This restricted, inducible expression pattern makes CD70 an attractive target for both agonist and blocking strategies.

CD27 Ligand, TNFSF7, Tumor Necrosis Factor Ligand 8A, CD27L, CD70 Antigen, TAN1 7, TAN17

TAN1-7

Clone TAN 1-7 recognizes mouse CD70.

This monoclonal antibody was purified using Protein G

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

ELISA, Flow Cytometry, Immunofluorescence, Blocking

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone TAN1-7 suitable for in vivo CD70 studies?

A: Yes - TAN1-7 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD70 blocking and in vitro work.

Q: Can I order anti-mouse CD70 clone TAN1-7 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone TAN1-7 target?

A: It is an anti-mouse CD70 monoclonal used to study and block CD70-CD27 costimulatory signalling; for research use only.