Anti-Mouse CD40 Antibody (FGK4.5) – APC
Product Benefits:
ichorbio’s Anti-Mouse CD40 Antibody (FGK4.5) – APC is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Target:
CD40
Clone:
FGK4.5
Isotype:
Rat IgG2a
Other Names:
CD40, FGK45
Uniprot:
Host:
Rat
Species Reactivity:
Mouse, Rat
Specificity:
Clone FGK4.5 recognizes an epitope on Mouse CD40
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
B cells, basal epithelial cells, macrophages, follicular dendritic cells, and endothelial cells
Background:
CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Immunogen:
Recombinant Mouse CD40 Fusion Protein
Concentration:
0.2 mg/ml
Formulation:
This APC conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage:
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Applications:
Flow Cytometry
Application Notes:
The suggested concentration for this FGK4.5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Reviews
There are no reviews yet.