Anti-Mouse CD370 (1F6) In Vivo Antibody - Low Endotoxin

Mouse

CLEC9A

>1mg/ml

Rat IgG1

Rat

Aggregation level ≤ 5%

RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope.

CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q8BRU4

Blue ice

This is clone 1F6, a rat IgG1 monoclonal raised against mouse CD370 (CLEC9A/DNGR-1, UniProt Q8BRU4), produced in a low-endotoxin, in-vivo-ready format supplied in bulk milligram-to-gram quantities for research use only. As a rat IgG1 it carries a relatively low-effector Fc backbone, which makes it well suited to receptor-engagement and antigen-targeting studies rather than aggressive Fc-mediated depletion, though effector contribution should always be confirmed empirically in the chosen model. 1F6 recognizes both the short and long stalk isoforms of mouse DNGR-1 and is a well-characterized tool for identifying and marking the CLEC9A-positive conventional type 1 dendritic cell (cDC1) lineage. Validated by the vendor for flow cytometry, immunofluorescence and Western blot, it is also widely applied in functional in-vivo work exploiting the highly restricted expression of CLEC9A on cDC1s, including DC-targeted antigen-delivery and immunization strategies. The low-endotoxin, high-purity preparation is intended to minimize confounding innate stimulation in animal experiments. All content here is provided as guidance for ichorbio to review before publishing. CD370, better known as CLEC9A or DNGR-1, is a group V C-type lectin receptor encoded by Clec9a and expressed as a disulfide-linked homodimer. Its expression is highly restricted, marking conventional type 1 dendritic cells (cDC1s) and, in mouse, a subset of plasmacytoid DCs and DC precursors, which makes it one of the most selective surface markers of the cross-presenting DC lineage. CLEC9A functions as a damage sensor: it binds filamentous actin (F-actin) exposed on necrotic and dead cells, whose membranes have lost integrity. Ligand engagement drives Syk-dependent signaling through a hemITAM motif in the cytoplasmic tail, promoting efficient cross-presentation of dead-cell-associated antigens to CD8+ T cells. This positions CLEC9A at the interface of tissue damage sensing and adaptive immune priming, and its restricted expression has made it an attractive receptor for DC-targeted vaccine and immunotherapy approaches.

CD370, DNGR-1, C-Type Lectin Domain Family 9 Member A

1F6

Clone 1F6 recognizes an epitope on mouse DNGR1

This monoclonal antibody was purified using Protein G

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Immunofluorescence, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 1F6 suitable for in vivo CLEC9A studies?

A: Yes - 1F6 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CLEC9A studies and in vitro work.

Q: Can I order anti-CD370 clone 1F6 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone 1F6 target?

A: It is a monoclonal against CLEC9A (CD370), a C-type lectin receptor on dendritic cells; for research use only.