Anti-Mouse CD318 (9a2) In Vivo Antibody - Low Endotoxin

Human/Mouse

CDCP1

>1mg/ml

Mouse IgG2b Kappa

Mouse

Aggregation level ≤ 5%

CDCP1 is widely expressed in the esophagus, skin, colon, duodenum, stomach and other tissues.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q9H5V8

Blue ice

This is a mouse monoclonal antibody (clone 9a2, mouse IgG2b kappa) raised against CDCP1 (CD318). It is supplied as a low-endotoxin, in-vivo-ready preparation intended for functional and animal studies rather than routine diagnostic work. The IgG2b kappa format and defined clonal identity give lot-to-lot consistency, which matters when a reagent is used across longitudinal in-vivo cohorts. Low endotoxin content (research grade <1 EU/mg; ultra-low <0.5 EU/mg) reduces the risk of non-specific immune activation that can confound results when antibody is delivered systemically at milligram doses. The product is offered in bulk (mg to gram) quantities to support dose-ranging and multi-arm study designs, and is labelled for research use only. On this card the validated application is flow cytometry, where clone 9a2 can be used to detect and quantify CDCP1 surface expression on cell populations. Researchers typically locate this reagent by its clone designation (9a2). As with all in-vivo antibodies, suitability for a given model should be confirmed empirically by the investigator. CDCP1 (CUB domain-containing protein 1), also known as CD318, SIMA135 or Trask, is a heavily glycosylated type I transmembrane glycoprotein (UniProt Q9H5V8). Its extracellular region contains CUB domains involved in cell-cell and cell-matrix interactions, and its cytoplasmic tail carries tyrosine residues phosphorylated by Src-family kinases. CDCP1 is expressed on subsets of epithelial, stem/progenitor and haematopoietic cells and is frequently upregulated in carcinomas. Proteolytic cleavage of the ectodomain generates fragments that modulate its signalling and adhesion functions. Through Src and PKC-delta engagement it influences cell adhesion, anoikis resistance, migration and invasion, and it has been studied as a marker and functional contributor in tumour progression and metastasis. Because of this restricted, context-dependent expression, CDCP1 is of interest both as a cell-surface identification marker and as a potential therapeutic target in oncology research.

CD318, CUB domain-containing protein 1, SIMA135

9a2

Clone 9A2 recognizes an epitope within the extracellular domain of human/mouse CDCP1

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 9a2 suitable for in vivo CDCP1 studies?

A: Yes - 9a2 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CDCP1 studies and in vitro work.

Q: Can I order anti-CD318 clone 9a2 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone 9a2 target?

A: It is a monoclonal antibody against CDCP1 (CD318) used to study this cell-surface glycoprotein; for research use only.