Anti-Mouse Ly-6G In Vivo Antibody – Low Endotoxin (1A8) [ICH1089]
$320.00 – $2,378.00
Bulk anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8)
ichorbio’s anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Ly-6G
1A8
Rat IgG2a
Lymphocyte antigen 6 complex, locus G
Rat
Mouse
Anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Mouse Ly-6G transfected EL-4J cell line
1.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
IHC (FFPE), IHC (Frozen), Flow Cytometry, Western Blot, In vivo Depletion, Immunfluorescence
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)
Anti-Ly-6G/Ly-6C In Vivo Antibody – Low Endotoxin [RB6-8C5] (ICH1131), Anti-Ly-6G/Ly-6C In Vivo Antibody – Ultra Low Endotoxin [RB6-8C5] (ICH1131UL), Anti-Ly-6C In Vivo Antibody – Low Endotoxin [HK1.4] (ICH1111), Anti-Ly-6C In Vivo Antibody – Low Endotoxin [7B10] (ICH1095)
Protocol:
1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose;
2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS;
3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h;
4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio Ly-6G antibody clone 1A8 (low) 1.3μg/ml, Final concentration (high) 6.5μg/ml. Positive signals were detected at both high and low concentrations
5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h
Details of secondary antibody:
Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody – Jackson Immunoresearch Labs Cat# 112-605-062 – Conc. 7.5μg/ml.
6. Washed by PBST at least 6 times；
7. Add fluorescence decay resistant medium, seal slice；
8. Detected by the laser scanning confocal microscope.
Scale bar in the IF figure is 50 μM.
Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University
Related products
Reviews
There are no reviews yet.