Bulk anti-iNKT cell In Vivo Antibody – Low Endotoxin (6B11)
Product Benefits:
ichorbio’s anti-iNKT cell In Vivo Antibody – Low Endotoxin (6B11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
iNKT cell
Clone:
6B11
Isotype:
Mouse IgG1
Other Names:
TCR Valpha24-Jalpha18, TCR Valpha24-JalphaQ, invariant NKT cell, iNKT
Host:
Mouse
Species Reactivity:
Human
Specificity:
Anti-iNKT cell In Vivo Antibody – Low Endotoxin (6B11) recognizes an epitope on TCR V alpha 24-J alpha 18
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Invariant NKT cells
Background:
Encoded by the TCR V alpha 24-J alpha 18 germline configuration, V alpha 24-J alpha Q is expressed on a subset of NKT cells, namely invariant NKT (iNKT). V alpha 24-J alpha Q TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production.
Immunogen:
Unknown
Concentration:
1.0 – 5.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-iNKT cell In Vivo Antibody – Low Endotoxin (6B11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Flow Cytometry
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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