Anti-Mouse IL-1a In Vivo Antibody – Low Endotoxin (ALF-161) [ICH1099]
$100.00 – $2,098.00
Bulk anti-IL-1a In Vivo Antibody – Low Endotoxin (ALF-161)
Product Benefits:
ichorbio’s anti-IL-1a In Vivo Antibody – Low Endotoxin (ALF-161) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
IL-1a
Clone:
ALF-161
Isotype:
Armenian Hamster IgG1
Other Names:
Interleukin-1 alpha, IL-1a
Uniprot:
Host:
Armenian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-IL-1a In Vivo Antibody – Low Endotoxin (ALF-161) recognizes Mouse IL-1A
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
Mouse IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-IL-1a In Vivo Antibody – Low Endotoxin (ALF-161) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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