Bulk anti-H-2K In Vivo Antibody – Low Endotoxin (Y-3)
ichorbio’s anti-H-2K In Vivo Antibody – Low Endotoxin (Y-3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
MHC Class 2
Anti-H-2K In Vivo Antibody – Low Endotoxin (Y-3) recognizes/react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ks and not H-2Kd MHC class I alloantigens
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
This Y-3 monoclonal antibody is reported to react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ksand not H-2Kd MHC class I alloantigens.
Con A stimulated spleen cells from BALB.B mice
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-H-2K In Vivo Antibody – Low Endotoxin (Y-3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Western Blot, Functional Assays
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.