Anti-Mouse CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) [ICH1085]
$320.00 – $2,378.00
Bulk anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11)
Bio X Cell:
ICH1085 is up to 11% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent product BE0032 from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE0032 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
Product Benefits:
ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CTLA-4
Clone:
UC10-4F10-11
Size:
ichorbio’s UC10-4F10-11 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 UC10-4F10-11 clone – please contact us for pricing.
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 UC10-4F10-11 clone – please contact us for pricing.
Isotype:
Armenian Hamster IgG1
Other Names:
Ctla4, Cytotoxic T-lymphocyte protein 4, CD152
Uniprot:
Host:
Armenian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) recognizes an epitope on Mouse CD152
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor.
Background:
CD152, also known as CTLA-4 or Ly-56 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Immunogen:
Purified Recombinant Mouse CTLA4 Fc Fusion Protein
Concentration:
≥ 2.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) is stable when stored at 2-8°C. Do not freeze.
Applications:
Flow Cytometry, Western Blot, Functional Assays, Immunoprecipitation
in vivo
We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone UC10-4F10-11. This range is based off the most recent publication data using the UC10-4F10-11 clone in vivo.
Application Notes:
Functional Assay: Clone UC10-4F10-11 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.
Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibodies against the same target:
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