ichorbio’s Anti-Mouse CD80 Antibody (16-10A1) – Biotin is manufactured in a cGMP compliant facility. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information.
Anti-CD80 Antibody (16-10A1) – Biotin recognizes an epitope on Mouse CD80
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.
CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.
Purified Recombinant Mouse CD80 (>98%)
This Biotin conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD80. A suggested method would be to stain cells expressing CD80 with 0.25 µg per 1 x 10^6 cells in a total staining volume of 100 µl followed by avidin. Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.