Bulk anti-CD45 In Vivo Antibody – Low Endotoxin (I3/2.3)
ichorbio’s anti-CD45 In Vivo Antibody – Low Endotoxin (I3/2.3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Anti-CD45 In Vivo Antibody – Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA). CD45 plays a key role in TCR and BCR signal transduction.
Mouse lymphoma cell line
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-CD45 In Vivo Antibody – Low Endotoxin (I3/2.3) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Western Blot, Flow Cytometry, IHC (Frozen), IHC (Paraffin)
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.