Anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) [ICH1042]
$320.00 – $2,378.00
Bulk anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5)
Bio X Cell:
ICH1042 is up to 19% cheaper for academia & non-profits and up to 52% cheaper for industry than the equivalent products BE0003-1 (low endotoxin) and BP0003-1 (ultra-low endotoxin) from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE/BP0003-1 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
Product Benefits:
ichorbio’s anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD4
Clone:
GK1.5
Size:
ichorbio’s GK1.5 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD4 GK1.5 clone – please contact us for pricing.
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD4 GK1.5 clone – please contact us for pricing.
Isotype:
Rat IgG2b
Other Names:
T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
Host:
Rat
Species Reactivity:
Mouse
Specificity:
Anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
Immunogen:
Mouse CTL clone V4
Concentration:
≥ 2.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Storage:
anti-CD4 In Vivo Antibody – Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Application Notes:
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.
Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Related Antibody:
Antibodies against the same target:
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