Anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) [ICH1040]
$320.00 – $2,378.00
Bulk anti-CD3e In Vivo Antibody – Low Endotoxin (500A2)
Product Benefits:
ichorbio’s anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD3e
Clone:
500A2
Isotype:
Syrian Hamster IgG2a
Other Names:
T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Host:
Syrian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
Background:
CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.
Immunogen:
Mouse T-cell receptor
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
<1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Storage:
anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Functional Assays, Flow Cytometry, IHC (Frozen)
Application Notes:
Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Related Antibody:
Antibodies against the same target:
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