Anti-Mouse CD3 In Vivo Antibody – Low Endotoxin (17A2) [ICH1039]
$320.00 – $2,140.00
Bulk anti-CD3 In Vivo Antibody – Low Endotoxin (17A2)
Bio X Cell:
ICH1039 is up to 11% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent products BE0002 (low endotoxin) from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE0002 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
Product Benefits:
ichorbio’s anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD3
Clone:
17A2
Size:
ichorbio’s 17A2 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD3 17A2 clone – please contact us for pricing.
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD3 17A2 clone – please contact us for pricing.
Isotype:
Rat IgG2b
Other Names:
T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e, Cd3g, T3 gamma chain, CD3 gamma chain
Host:
Rat
Species Reactivity:
Mouse
Specificity:
Anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) recognizes an epitope on Mouse CD3. The 17A2 clone reacts with the epsilon and gamma chains and not delta nor zeta of CD3.
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
Background:
CD3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR A/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Immunogen:
gamma/delta TCR-positive T-T hybridoma D1
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen), Immunocytochemistry
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Antibodies against the same target:
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