Anti-Mouse CD172a In Vivo Antibody – Low Endotoxin (P84) [ICH1076]
$320.00 – $2,378.00
Bulk anti-CD172a In Vivo Antibody – Low Endotoxin (P84)
Product Benefits:
ichorbio’s anti-CD172a In Vivo Antibody – Low Endotoxin (P84) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD172a
Clone:
P84
Isotype:
Rat IgG1
Other Names:
Sirpa, Sirp-alpha-1, SHP substrate 1, SHPS-1, Shps1, Bit, p84, MyD-1 antigen, Myd1, Ptpns1
Uniprot:
Host:
Rat
Species Reactivity:
Mouse, Rat
Specificity:
Anti-CD172a In Vivo Antibody – Low Endotoxin (P84) recognizes an epitope on Mouse CD172a
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Monocytes, macrophages, myeloid cells, neuronal tissue
Background:
Signal regulatory protein alpha (SIRPalpha), also known as CD172a, is a regulatory membrane type I glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.
Immunogen:
Mouse brain membrane protein
Concentration:
1.0 – 5.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD172a In Vivo Antibody – Low Endotoxin (P84) is stable when stored at 2-8°C. Do not freeze.
Applications:
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Application Notes:
Blocking: Reported applications in literature include: blocking SIRPalpha interaction with CD47 & in vivo blocking of dendritic cell migration.
Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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