Anti-Human CD16 In Vivo Antibody – Low Endotoxin (3G8) [ICH1010]
$320.00 – $2,140.00
Bulk anti-CD16 In Vivo Antibody – Low Endotoxin (3G8)
Product Benefits:
ichorbio’s anti-CD16 In Vivo Antibody – Low Endotoxin (3G8) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD16
Clone:
3G8
Isotype:
Mouse IgG1
Other Names:
FCGR3A, Fc-gamma RIII, FcRIII
Uniprot:
Host:
Mouse
Species Reactivity:
Human
Specificity:
Anti-CD16 In Vivo Antibody – Low Endotoxin (3G8) recognizes an epitope on Human CD16
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.
Background:
CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.
Immunogen:
Human PMN cells
Concentration:
≥ 2.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD16 In Vivo Antibody – Low Endotoxin (3G8) is stable when stored at 2-8°C.
Applications:
IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, Flow Cytometry, CyTOF
Application Notes:
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume.
Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation.
Each investigator should determine their own optimal working dilution for specific applications.
Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation.
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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Isotype Controls
Rat IgG2a In Vivo Isotype Control – Low Endotoxin (1-1) [ICH2244]
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