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Home / Applications / Blocking
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Anti-Mouse CD126 In Vivo Antibody – Low Endotoxin (15A7) [ICH1104]

$184.00 – $313.00

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Anti-Mouse CD126 In Vivo Antibody – Low Endotoxin (15A7) [ICH1104]

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SKU: ICH1104 Categories: anti-mouse, Blocking, Functional Assays, Low Endotoxin, Western Blot
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Bulk anti-CD126 In Vivo Antibody – Low Endotoxin (15A7)

 

Product Benefits:
ichorbio’s anti-CD126 In Vivo Antibody – Low Endotoxin (15A7) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD126
Clone:
15A7
Isotype:
Rat IgG2b
Other Names:
Interleukin-6 receptor subunit alpha, Il6ra, IL-6R-alpha, IL-6RA
Uniprot:
P22272
Host:
Rat
Species Reactivity:
Mouse
Specificity:
Anti-CD126 In Vivo Antibody – Low Endotoxin (15A7) recognizes an epitope on Mouse CD126. The D7715A7 (Clone 15A7) antibody blocks IL-6/IL-6 receptor interactions
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Activated T and B cells, monocytes, hepatocytes, plasma cells
Background:
CD126 is an 80 kD IL-6 receptor alpha chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor beta chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The beta chain does not bind IL-6 by itself, but associates with the alpha-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation.
Immunogen:
OKT4 hybridoma cells
Concentration:
1.0 – 5.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:
anti-CD126 In Vivo Antibody – Low Endotoxin (15A7) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Functional Assays, Blocking
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Isotype Control:
Rat IgG2b In Vivo Isotype Control – Low Endotoxin [1-2] (ICH2243)
Size:

0.5mg, 1mg

Endotoxin Level:

Low, Ultra low

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About us

From Oxford, United Kingdom - ichorbio supplies the best antibodies for in vivo research. Our in vivo antibodies are sold for the purposes of research only, not for therapeutic or diagnostic use.

ichorbio Ltd, Grove Business Park, Downsview Road, Wantage OX12 9FF

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