Anti-Mouse Integrin Beta 7 (FIB504) In Vivo Antibody - Low Endotoxin

Mouse

ITGB7

>1mg/ml

Rat IgG2a Kappa

Rat

Anti-Mouse integrin β7 (FIB21) In Vivo Antibody - Low Endotoxin (ICH1234)

Aggregation level ≤ 5%

TK1 Cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P26011

Blue ice

This product is a purified rat anti-mouse Integrin beta 7 (ITGB7) monoclonal antibody, clone FIB504, of the rat IgG2a kappa isotype. FIB504 is a widely used, well-characterized clone that recognizes the mouse beta 7 integrin subunit and blocks its adhesive function, making it suited to in-vivo and functional studies of lymphocyte trafficking and gut/mucosal homing rather than purely descriptive work. The antibody is validated for flow cytometry and for functional blocking applications. It is manufactured to a low-endotoxin specification (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and supplied in bulk milligram-to-gram quantities, which supports repeated dosing across treatment groups and longer in-vivo studies where endotoxin contamination would otherwise confound immune readouts. As a rat IgG2a, it pairs naturally with a matched rat IgG2a isotype control for in-vivo experiments. All material is for research use only (RUO). Researchers typically identify this reagent by its clone designation, FIB504, when comparing lots and vendors. Integrin beta 7 (ITGB7, UniProt P26011) is an integrin beta subunit that pairs with alpha chains to form two heterodimers central to mucosal immunity. With alpha 4 (ITGA4) it forms alpha4beta7 (LPAM-1), the gut-homing integrin that binds MAdCAM-1 on intestinal and gut-associated lymphoid endothelium, directing lymphocyte trafficking to the gut and Peyer's patches. With alpha E (CD103, ITGAE) it forms alphaEbeta7, which binds E-cadherin on epithelial cells and retains intraepithelial lymphocytes and tissue-resident memory T cells within mucosal epithelium. Because beta 7 is shared by both heterodimers, it is a key determinant of intestinal immune homeostasis, oral tolerance, and mucosal inflammation. The alpha4beta7/MAdCAM-1 axis is a validated therapeutic target in inflammatory bowel disease, and beta 7 also contributes to lymphocyte retention in tissues such as the intestinal lamina propria.

Integrin beta-7, Integrin beta-P, M290 IEL antigen

FIB504

Anti-integrin beta 7 In Vivo Antibody - Low Endotoxin (FIB504) recognizes an epitope on Mouse Integrin B7

This monoclonal antibody was purified using Protein G

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-integrin beta 7 In Vivo Antibody - Low Endotoxin (FIB504) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycle

Flow Cytometry, Blocking

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone FIB504 suitable for in vivo use?

A: Yes — FIB504 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo ITGB7 studies as well as in vitro work.

Q: Can I order FIB504 in bulk or gram quantities?

A: Yes, this anti-mouse ITGB7 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone FIB504 target?

A: It is an anti-mouse ITGB7 monoclonal antibody that binds ITGB7 and is used in functional and blocking studies; for research use only (RUO).