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Anti-Mouse TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) [ICH1136]
$320.00 – $2,378.00
Bulk anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12)
Bio X Cell:
ICH1136 is up to 11% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent product BE0244 from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE0244 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
Product Benefits:
ichorbio’s anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
TNF alpha
Clone:
TN3-19.12
Size:
ichorbio’s TN3-19.12 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-TNF alpha TN3-19.12 clone – please contact us for pricing.
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-TNF alpha TN3-19.12 clone – please contact us for pricing.
Isotype:
Armenian Hamster IgG
Other Names:
Cachectin, TNFa, Tumor necrosis factor ligand superfamily member 2, Tnfsf2
Uniprot:
Host:
Armenian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) recognizes Mouse TNFa
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Background:
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha
Immunogen:
Recombinant mouse TNFalpha
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Storage:
anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications:
Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, Blocking
How much TN3-19.12 to use in vivo:
We recommend using 250 µg per mouse when performing <i>in vivo</i> research using ichorbio’s low endotoxin TNF alpha antibody clone TN3-19.12. This range is based off the most recent publication data using the TN3-19.12 clone <i>in vivo</i>. Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Related Antibody:
Antibodies against the same target:
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