Anti-Mouse MHC Class I (A4C8.1-Do9) In Vivo Antibody - Low Endotoxin

Mouse

MHC Class I

>1mg/ml

Mouse IgG1 Kappa

Mouse

Bulk Anti-Human MHC Class I (W6/32) Antibody (ICH1023), Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin (ICH1203), Anti-Mouse MHC Class I, H2 (M1/42.3.9.8) In Vivo Antibody - Low Endotoxin (ICH1279)

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01901

14972

RRID:AB_2921481

Blue ice

This is clone A4C8.1-Do9, a mouse IgG1 kappa monoclonal antibody raised against mouse MHC Class I (H-2, UniProt P01901). Because it recognises a self antigen and is produced as an intact mouse IgG1, it is a useful reagent for studying MHC Class I expression, regulation and surface presentation in mouse tissues and cell lines. This preparation is supplied low-endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg options) and is available in bulk milligram-to-gram quantities, making it suitable for larger studies where lot-to-lot consistency and low endotoxin matter. The listed validated application is Western Blot, where the clone detects denatured MHC Class I heavy chain. Researchers typically identify this reagent by its clone designation, A4C8.1-Do9. As with all products in this range, it is supplied for research use only (RUO) and is not for diagnostic or therapeutic use. The antibody is intended as a research tool for characterising MHC Class I biology in mouse experimental systems, and all content here is guidance for review before publication. MHC Class I molecules are cell-surface glycoproteins that present intracellularly derived peptide antigens to CD8+ cytotoxic T lymphocytes, forming a central axis of adaptive immune surveillance. The complete complex comprises a polymorphic heavy chain (encoded in the mouse H-2 region; UniProt P01901 corresponds to the H2-K1 heavy chain) non-covalently associated with beta-2 microglobulin and a short peptide typically 8-10 residues long, loaded in the endoplasmic reticulum via the peptide-loading complex. Expressed on virtually all nucleated cells, MHC Class I allows CD8+ T cells to detect viral infection and malignant transformation, while its engagement of inhibitory receptors (such as Ly49 and KIR family members) restrains NK cell killing under the "missing-self" principle. Downregulation of MHC Class I is a common tumour and viral immune-evasion strategy, making it a frequent readout in immuno-oncology and infection studies.

Class I H-2Kd, A4C8 1-Do9, A4C8-1-Do9, A4C8.1 Do9, A4C8.1Do9, A4C81-Do9, A4C81Do9

A4C8.1-Do9

Anti-MHC Class I In Vivo Antibody - Low Endotoxin (A4C8.1-Do9) recognizes an epitope on Mouse Class I H-2Kd

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-MHC Class I In Vivo Antibody - Low Endotoxin (A4C8.1-Do9) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycle

Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Mouse MHC Class I/A4C8-1-Do9 (A4C8.1-Do9) In Vivo Antibody - Low Endotoxin

Q: Is clone A4C8.1-Do9 suitable for in vivo use?

A: Yes — A4C8.1-Do9 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo MHC Class I studies as well as in vitro work.

Q: Can I order A4C8.1-Do9 in bulk or gram quantities?

A: Yes, this anti-mouse MHC Class I antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone A4C8.1-Do9 target?

A: It is an anti-mouse MHC Class I monoclonal antibody that binds MHC Class I and is used in functional and blocking studies; for research use only (RUO).