Anti-Mouse IL-1a (ALF-161) In Vivo Antibody - Low Endotoxin

Mouse

IL-1a

>1mg/ml

Armenian Hamster IgG1

Armenian Hamster

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01582

Blue ice

This product is clone ALF-161, an Armenian Hamster IgG1 monoclonal antibody raised against mouse IL-1a (interleukin-1 alpha, UniProt P01582). As a hamster anti-mouse reagent, it is well suited to in-vivo neutralization studies and functional work in mouse models, where it binds IL-1a and blocks its interaction with the IL-1 receptor. ALF-161 is widely used for in-vivo IL-1a neutralization and across in-vitro applications including Western blot, ELISA, ELISPOT, immunoprecipitation, and intracellular flow cytometry. This in-vivo formulation is manufactured to low endotoxin levels (research grade typically less than 1 EU/mg; ultra-low options less than 0.5 EU/mg) to minimize confounding innate immune activation during systemic administration. It is supplied in bulk quantities (milligram to gram scale) suitable for repeated-dosing regimens and larger animal cohorts. All material is for research use only (RUO). Researchers commonly identify this reagent by its clone designation, ALF-161, when comparing across suppliers. IL-1a (interleukin-1 alpha) is a pro-inflammatory cytokine of the IL-1 family and, with IL-1b, is one of the two principal IL-1 receptor agonists. Unlike IL-1b, IL-1a is constitutively expressed by many cell types (epithelial cells, keratinocytes, fibroblasts, endothelial cells) and is present as a precursor that is biologically active without caspase-1 processing. It localizes to the cytosol and nucleus and can be released upon cell death or necrosis, acting as an alarmin (DAMP) that signals tissue damage. Both membrane-associated and secreted forms engage the type I IL-1 receptor (IL-1R1) together with the co-receptor IL-1RAcP, driving MyD88/NF-kB signaling and downstream inflammation. IL-1a contributes to sterile inflammation, fever, leukocyte recruitment, and tumor-associated inflammation, making it a target of interest in inflammatory disease and cancer models.

Interleukin-1 alpha, IL-1a, ALF 161, ALF161

ALF-161

Anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) recognizes Mouse IL-1A

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeate

Western Blot, ELISA, ELISPOT, Neutralization, Immunoprecipitation, Flow Cytometry (Intracellular)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone ALF-161 suitable for in vivo use?

A: Yes — ALF-161 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo IL-1a studies as well as in vitro work.

Q: Can I order ALF-161 in bulk or gram quantities?

A: Yes, this anti-mouse IL-1a antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone ALF-161 target?

A: It is an anti-mouse IL-1a monoclonal antibody that binds IL-1a and is used in functional and blocking studies; for research use only (RUO).