Anti-Mouse IL-1R (JAMA-147) In Vivo Antibody - Low Endotoxin

Mouse

IL1R1

>1mg/ml

Armenian Hamster IgG

Armenian Hamster

Anakinra Biosimilar - Research Grade (ICH5343)

Aggregation level ≤ 5%

Extracellular domain of mouse IL-1 R type 1

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P13504

Blue ice

This product is JAMA-147, an Armenian hamster IgG monoclonal antibody raised against mouse IL-1R1 (interleukin-1 receptor type 1, UniProt P13504). Because it is a hamster anti-mouse reagent that binds the receptor directly, it is well suited to functional and in-vivo studies in mouse models where blocking IL-1 signalling is the goal, and it also supports immunoprecipitation, western blot, and flow cytometry for target detection and validation. Hamster IgG framework antibodies are widely used in mouse work because they are recognised as foreign only weakly, allowing repeat dosing while limiting confounding host immune responses. The preparation is manufactured to low-endotoxin specifications (research grade under 1 EU/mg; ultra-low grade under 0.5 EU/mg), which is important for in-vivo experiments where contaminating endotoxin would itself trigger innate immune and IL-1-family responses and confound results. It is available in bulk milligram-to-gram quantities to support dosing across cohorts and time-course designs. Supplied for research use only (RUO). All content here is guidance for ichorbio to review before publishing. IL-1R1 (IL1R1, CD121a; UniProt P13504) is the primary signalling receptor for the interleukin-1 family cytokines IL-1 alpha and IL-1 beta. It is a type I transmembrane glycoprotein with three extracellular immunoglobulin-like domains and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Productive signalling requires ligand-induced recruitment of the accessory protein IL-1RAcP (IL1RAP), which assembles a ternary complex that engages the adaptor MyD88, IRAK kinases, and TRAF6, driving activation of NF-kappaB and MAPK pathways and downstream pro-inflammatory gene expression. Signalling is naturally restrained by the secreted decoy receptor IL-1R2 and by the endogenous antagonist IL-1Ra. IL-1R1 is broadly expressed, including on fibroblasts, endothelial and epithelial cells, and subsets of immune cells, and its activity is central to sterile and infectious inflammation, fever, and autoinflammatory disease. It is therefore a frequent target in inflammation, autoimmunity, and tumour microenvironment research.

Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, IL-1R-alpha, Interleukin-1 receptor type I, p80, CD121a, JAMA 147, JAMA147

JAMA-147

Anti-IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) recognizes Mouse IL-1 R1

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-IL-1R In Vivo Antibody - Low Endotoxin (JAMA-147) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeat

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone JAMA-147 suitable for in vivo use?

A: Yes — JAMA-147 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo IL1R1 studies as well as in vitro work.

Q: Can I order JAMA-147 in bulk or gram quantities?

A: Yes, this anti-mouse IL1R1 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone JAMA-147 target?

A: It is an anti-mouse IL1R1 monoclonal antibody that binds IL1R1 and is used in functional and blocking studies; for research use only (RUO).