Anti-Mouse H-2K (Y-3) In Vivo Antibody - Low Endotoxin

Mouse

H-2K

>1mg/ml

Mouse IgG2b Kappa

Rat

Aggregation level ≤ 5%

Con A stimulated spleen cells from BALB.B mice

This Y-3 monoclonal antibody is reported to react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ksand not H-2Kd MHC class I alloantigens.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01901;P04223;P01902

RRID:AB_2921506

Blue ice

This is clone Y-3, an antibody directed against mouse H-2K, a classical MHC class I molecule. The Y-3 clone was originally raised by immunising a BALB/c (H-2d) mouse with concanavalin-A-stimulated spleen cells from the congenic BALB.B (H-2b) strain, and it reacts with H-2K across several haplotypes (b, k, q, r, s) while not binding H-2d. The clone is well established in immunology as a tool for identifying, blocking and immunoprecipitating H-2K class I heavy chains. ichorbio supplies it in an in-vivo-ready format: purified, low endotoxin (<1 EU/mg research grade, with an ultra-low <0.5 EU/mg option), and available in bulk milligram-to-gram quantities suited to repeat-dosing mouse studies. Validated applications include Western blot and functional/blocking assays. These attributes make it appropriate for functional MHC class I work in vivo and in vitro, such as blocking CD8 T-cell recognition, transplantation and allorecognition models, and cell-surface class I detection. For research use only; not for diagnostic or therapeutic use. H-2K is one of the two classical MHC class I loci in the mouse (alongside H-2D), the murine counterpart of human HLA class I. It encodes a polymorphic heavy chain that pairs non-covalently with beta-2 microglobulin and presents short (typically 8-10 residue) endogenously derived peptides at the cell surface. This peptide-MHC complex is surveyed by the T-cell receptor of CD8 cytotoxic T lymphocytes, allowing the immune system to detect intracellular pathogens and tumour-associated antigens. H-2K is highly polymorphic between mouse strains, defining haplotypes (e.g. H-2Kb in C57BL/6), which underlies its central role in allorecognition and transplant rejection. Class I molecules also serve as ligands for inhibitory and activating NK-cell receptors, contributing to "missing-self" recognition. Because it is expressed on nearly all nucleated cells, H-2K is a widely used marker of MHC class I biology and antigen presentation.

MHC Class 2, H2-K1, histocompatibility 2, K1, K region, Y 3, Y3

Y-3

Anti-H-2K In Vivo Antibody - Low Endotoxin (Y-3) recognizes/react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ks and not H-2Kd MHC class I alloantigens

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-H-2K In Vivo Antibody - Low Endotoxin (Y-3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0172

Anti-Mouse H-2K/H2-K1 (Y-3) In Vivo Antibody - Low Endotoxin

Q: Is clone Y-3 suitable for in vivo H-2K studies?

A: Yes - Y-3 is a low-endotoxin, in vivo-grade antibody suitable for in vivo H-2K studies and in vitro work.

Q: Can I order anti-mouse H-2K clone Y-3 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone Y-3 target?

A: It is an anti-mouse H-2K monoclonal used to study MHC class I expression and function; for research use only.