Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) [ICH1084]
$320.00 – $2,378.00
Bulk anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10)
Bio X Cell:
ICH1084 is up to 26% cheaper for academia & non-profits and up to 58% cheaper for industry than the equivalent products BE0131 (low endotoxin) and BP0131 (ultra-low endotoxin) from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE/BP0131 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
Product Benefits:
ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CTLA-4
Clone:
9H10
Size:
ichorbio’s 9H10 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9H10 clone – please contact us for pricing.
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9H10 clone – please contact us for pricing.
Isotype:
Syrian Hamster IgG
Other Names:
Ctla4, Cytotoxic T-lymphocyte protein 4, CD152
Uniprot:
Host:
Syrian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) recognizes an epitope on Mouse CTLA-4
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Activated T cells
Background:
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. Clone 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA4 binding, allowing for CD28 binding.
Immunogen:
Mouse CTLA-4-human IgG1 fusion protein
Concentration:
1.0 – 5.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.5 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler’s murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
Storage:
anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Western Blot, Blocking, ELISA, Flow Cytometry
in vivo
We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9H10. This range is based off the most recent publication data using the 9H10 clone.
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications. A number of studies have used 100ug per injection for T-cell modulation in vivo.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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