Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Mouse

CD86

>1mg/ml

Rat IgG2a Kappa

Rat

Aggregation level ≤ 5%

LPS-activated CBA/Ca mouse splenic B cells

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P42082

Blue ice

This is clone GL1, a rat IgG2a kappa monoclonal antibody raised against mouse CD86 (B7-2), produced in rat host and validated across a broad range of applications including flow cytometry, immunoprecipitation, frozen-section IHC, immunocytochemistry, immunofluorescence, Western blot, CyTOF, and functional blocking. GL1 is one of the most widely referenced anti-mouse CD86 clones and recognises the native surface form on antigen-presenting cells, making it suitable for both phenotyping and functional in-vivo work. This ichor.bio preparation is offered as a low-endotoxin, research-grade format (typically <1 EU/mg, with an ultra-low <0.5 EU/mg option) and is available in bulk milligram-to-gram quantities, which supports repeated dosing across treatment cohorts and reproducible study designs. The low endotoxin load is important for in-vivo administration because contaminating LPS independently activates innate immune cells and confounds costimulation-focused experiments. Supplied for research use only (RUO). All content here is guidance for ichor.bio to review before publishing. CD86 (B7-2) is a costimulatory glycoprotein of the immunoglobulin superfamily expressed on activated antigen-presenting cells including dendritic cells, macrophages, and B cells, encoded in mouse by the gene corresponding to UniProt P42082. Together with its close relative CD80 (B7-1), CD86 engages two counter-receptors on T cells: CD28, which delivers the positive "signal 2" that amplifies TCR-driven activation, proliferation, and cytokine production; and CTLA-4, which delivers an inhibitory signal and competes for the same ligands with higher avidity. CD86 tends to be constitutively present at low levels and is rapidly upregulated upon APC activation, making it an early marker of dendritic-cell and B-cell maturation. Through this CD28/CTLA-4 axis, CD86 is central to shaping the balance between T-cell priming and peripheral tolerance, and it is a frequent readout and target in immunology, transplantation, and autoimmunity studies.

Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1

GL1

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

This monoclonal antibody was purified using Protein G

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone GL1 suitable for in vivo CD86 blocking?

A: Yes, GL1 is supplied in a low-endotoxin, in vivo-grade format suitable for CD86 blocking and functional studies as well as in vitro work.

Q: Can I order clone GL1 anti-CD86 in bulk?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput in vivo experiments.

Q: What does clone GL1 target?

A: GL1 is an anti-mouse CD86 (B7-2) monoclonal antibody used to study costimulation; for research use only.