Bulk Anti-Human CD8 (UCHT-4) Antibody

Human

CD8

>1mg/ml

Mouse IgG2a

Mouse

Anti-Mouse CD8 (YTS-169) In Vivo Antibody - Low Endotoxin (ICH1043), Anti-Mouse CD8 (116-13.1) In Vivo Antibody - Low Endotoxin (ICH1249), Crefmirlimab Biosimilar - Research Grade (ICH5453)

Aggregation level ≤ 5%

The CD8 antigen is expressed on approximately 19-48% of normal human peripheral blood lymphocytes and > 90% of normal thymocytes. It is also present on a subset of NK cells. Subjects infected with HIV have been shown to exhibit increased levels of CD8+ ly

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01732

925

Blue ice

This is a bulk-format mouse monoclonal antibody (clone UCHT-4, isotype Mouse IgG2a) raised against human CD8, the alpha chain of the CD8 co-receptor (UniProt P01732). Because it is a well-characterised anti-human CD8 clone, it is suited to identifying, quantifying, sorting and functionally probing human cytotoxic and CD8-positive T cells across a broad set of applications, including flow cytometry, Western blot, immunoprecipitation, IHC, immunofluorescence, ELISA and functional assays. Supplied as a research-use-only (RUO) reagent in bulk quantities (mg to gram scale), it is intended for laboratories that consume large amounts of a single validated lot and want lot-to-lot consistency for screening, assay development or reagent manufacturing workflows. Low-endotoxin formulation makes it appropriate for sensitive primary-cell and functional work where endotoxin contamination could confound results. As a human-reactive antibody, it is used primarily in vitro and ex vivo on human samples rather than in mouse in-vivo dosing studies. CD8 is a cell-surface glycoprotein co-receptor central to the cytotoxic arm of adaptive immunity. On conventional cytotoxic T lymphocytes it exists as an alpha/beta heterodimer, while CD8 alpha/alpha homodimers appear on subsets of NK cells, dendritic cells and intraepithelial lymphocytes. The CD8 co-receptor binds the largely invariant alpha-3 domain of MHC class I molecules, stabilising the interaction between the T-cell receptor and peptide-MHC class I on target cells. Through its cytoplasmic tail CD8 alpha recruits the Src-family kinase LCK, bringing this kinase into proximity with the TCR-CD3 complex to initiate proximal signalling and lower the threshold for T-cell activation. CD8 expression thus marks and enables the T cells responsible for recognising and killing virally infected and tumour cells, making it one of the most widely used lineage markers in human immunology and immuno-oncology.

T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain, CD8A, MAL, UCHT 4, UCHT4

UCHT-4

Anti-CD8 In Vivo Antibody - Low Endotoxin (UCHT-4) recognizes Human CD8. Anti-Human CD8 recognizes a T-cell glycoprotein cell surface complex (Mr 32 kDa) which defines the cytotoxic/suppressor population of mature T-lymphocytes. The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation. The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p56lck).

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD8 In Vivo Antibody - Low Endotoxin (UCHT-4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Western Blot, Immunoprecipitation, Functional Assays, IHC, Immunofluorescence, ELISA

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Human CD8/CD8A (UCHT-4) Antibody

Q: Can I order clone UCHT-4 anti-CD8 in bulk?

A: Yes, UCHT-4 is manufactured in scalable milligram to gram quantities to support bulk and high-throughput studies.

Q: Is clone UCHT-4 low endotoxin and in vivo ready?

A: Yes, it is supplied in a low-endotoxin, in vivo-suitable research-grade format for functional and in vitro use.

Q: What does clone UCHT-4 detect?

A: UCHT-4 is an anti-human CD8 monoclonal antibody used to identify cytotoxic T cells in flow cytometry and functional assays; for research use only.