Bulk Anti-Human CD16 (3G8) Antibody

Human

CD16

>1mg/ml

Mouse IgG1 Kappa

Mouse

Human CD16a (176F) Protein (In Vivo Grade, Ultra-Low Endotoxin) (ICH3014), Human CD16a (176V) Protein (In Vivo Grade, Ultra-Low Endotoxin) (ICH3015)

Aggregation level ≤ 5%

Human PMN cells

CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P08637

2214

Blue ice

This is clone 3G8, a mouse IgG1 kappa monoclonal antibody raised against human CD16 (FcgammaRIII), one of the most widely used and best-characterized anti-CD16 clones. It recognizes human CD16 and is validated for IHC on frozen sections, flow cytometry, immunoprecipitation, functional blocking, and CyTOF (mass cytometry), making it a versatile reagent for immunophenotyping and functional studies of NK cells, monocyte/macrophage subsets, and granulocytes. As a human-reactive antibody, it is suited to in-vitro and ex-vivo functional research rather than mouse in-vivo depletion or blockade models. Supplied in bulk (mg to gram scale) as a research-grade, low-endotoxin (<1 EU/mg) preparation, it supports demanding applications where high antibody mass, lot consistency, and low endotoxin matter, such as receptor-blocking assays, ADCC/CD16-crosslinking experiments, primary human cell isolation, and metal-conjugated CyTOF panels. For research use only (RUO). The 3G8 clone is a common benchmark, easing comparison with the published literature. CD16 is a low-affinity Fc receptor for IgG (FcgammaRIII) that binds the Fc region of IgG immune complexes and antibody-opsonized targets. It exists as two closely related genes: FcgammaRIIIa (CD16a, UniProt P08637), a transmembrane signaling receptor expressed on NK cells, monocytes, macrophages, and some T-cell subsets, and FcgammaRIIIb (CD16b), a GPI-anchored form on neutrophils. On NK cells, CD16a is the principal trigger of antibody-dependent cellular cytotoxicity (ADCC): Fc engagement drives signaling through associated CD3zeta and FcRgamma ITAM chains, promoting cytotoxic granule release and cytokine production. CD16a is subject to activation-induced shedding by ADAM17, which modulates NK effector function. A well-known FcgammaRIIIa polymorphism (V158F) alters IgG binding affinity and correlates with clinical responses to therapeutic antibodies, making CD16 central to studies of ADCC and antibody effector function.

FCGR3A, Fc-gamma RIII, FcRIII

3G8

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.

anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.

IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Human CD16/FCGR3A (3G8) Antibody

Q: Is clone 3G8 suitable for in vivo use?

A: Yes, 3G8 is supplied in a low-endotoxin, in vivo-grade format suitable for blocking and functional CD16 studies as well as in vitro work.

Q: Can I order clone 3G8 anti-CD16 in bulk?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone 3G8 target?

A: 3G8 is an anti-human CD16 monoclonal antibody used to identify NK cells and study Fc receptor function; for research use only.