Anti-Mouse CD155 (4.24) In Vivo Antibody - Low Endotoxin

Bulk anti-CD155 In Vivo Antibody - Low Endotoxin (4.24)

Product Benefits:

ichorbio's anti-CD155 In Vivo Antibody - Low Endotoxin (4.24) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD155

Clone:

4.24

Isotype:

Rat IgG2a

Other Names:

Pvr, Poliovirus receptor

Uniprot:

Q91WP1

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD155 In Vivo Antibody - Low Endotoxin (4.24) recognizes an epitope on Mouse CD155

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

CD155 is expressed at cell junctions on the primary vascular endothelial cells. Also, mouse CD155 is highly expressed on DP thymocytes.

Background:

Mouse CD155, is a member of a subfamily of immunoglobulin-like adhesion receptors. Mouse CD155 is expressed on a variety of cells of hematopoietic origin apart from its existence on the epithelial cells. The engagement of CD155 with CD226 and CD96 induces cytotoxicity of NK cells and CTL.

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Functional Assays, Flow Cytometry

How much 4.24 to use in vivo

We recommend using 250 µg per mouse when performing in vivo research using ichorbio’s CD155 low endotoxin antibody clone 4.24. This range is based off the most recent publication data using the 4.24 clone in vivo: contact us if you would like the latest publications.

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)