Anti-Mouse CD120a (55R-170) In Vivo Antibody - Low Endotoxin

Chuah S. et al. Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma. Journal of Hepatology, 2022, 77, 3: 683-694.

Mouse

CD120a

>1mg/ml

Armenian Hamster IgG1

Armenian Hamster

Anti-Mouse CD120a (55R-593) In Vivo Antibody - Low Endotoxin (ICH1069)

Aggregation level ≤ 5%

Recombinant Mouse TNF RI Extracellular Domain

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P25118

Blue ice

Anti-Mouse CD120a clone 55R-170 is an Armenian hamster IgG1 monoclonal raised against mouse CD120a (TNF receptor 1, TNFR1/p55). Armenian hamster IgG mAbs are widely used for in vivo work against mouse targets because they are well tolerated over repeated dosing and their non-mouse origin gives clean, low-background staining in mouse systems. This preparation is validated across immunoprecipitation, Western blot, flow cytometry, and functional/blocking and neutralization assays, making it suitable for both mechanistic characterization and receptor-level intervention studies. It is manufactured to a low-endotoxin research grade (<1 EU/mg; ultra-low <0.5 EU/mg) and supplied in bulk milligram-to-gram quantities, so that endotoxin-driven artefacts are minimized in sensitive in vivo TNF-signaling models where background inflammation must be controlled. The antibody is aggregate-screened and buffered without carrier proteins or preservatives that interfere with injection. It is provided for research use only (RUO). These attributes make clone 55R-170 appropriate for investigators dissecting TNFR1-dependent inflammation, cell death, and immune signaling in mouse models, and for repeated-dose in vivo receptor-blocking studies. CD120a, better known as tumor necrosis factor receptor 1 (TNFR1; also p55/p60), is encoded by Tnfrsf1a and is the principal receptor mediating TNF-alpha signaling. It is expressed broadly on most nucleated cell types, in contrast to the more restricted TNFR2 (CD120b). TNFR1 binds both soluble and membrane TNF and, uniquely among the two receptors, contains a cytoplasmic death domain. Ligand engagement triggers assembly of complex I (TRADD, TRAF2, RIPK1, cIAPs) that activates NF-kB and MAPK pathways to drive pro-inflammatory and pro-survival gene expression; under conditions of altered ubiquitination or checkpoint loss, signaling shifts to complex II, promoting caspase-8-dependent apoptosis or RIPK3/MLKL-driven necroptosis. Through these outputs TNFR1 governs inflammation, host defense, tissue homeostasis, and cell-fate decisions, and is central to models of septic shock, autoimmune and inflammatory disease, and inflammation-driven pathology.

Tumor necrosis factor receptor superfamily member 1A, Tnfrsf1a, TNF-R1, TNF-RI, p55, p60, 55R 170, 55R170

55R-170

Anti-CD120a In Vivo Antibody - Low Endotoxin (55R-170) recognizes an epitope on Mouse TNF RI

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD120a In Vivo Antibody - Low Endotoxin (55R-170) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeat

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, Neutralization

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 55R-170 suitable for in vivo use?

A: Yes — 55R-170 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD120a studies as well as in vitro work.

Q: Can I order 55R-170 in bulk or gram quantities?

A: Yes, this anti-mouse CD120a antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 55R-170 target?

A: It is an anti-mouse CD120a monoclonal antibody that binds CD120a and is used in functional and blocking studies; for research use only (RUO).